A20 Antibody / TNFAIP3

A20 (also known as TNFAIP3) is a zinc finger protein and inhibits NF-kappa B activation as well as TNF-mediated apoptosis. The protein has both ubiquitin ligase and deubiquitinase activities, is involved in the cytokine-mediated immune and inflammatory responses. A20 is as a tumor suppressor and has been shown to protect MCF-7 breast carcinoma cells from TNF-induced apoptosis. High A20 expression levels were observed in more aggressive breast tumors (ER-negative, progesterone receptor-negative and high histological grade) . These findings strongly suggest that A20 is a key protein involved in tamoxifen resistance.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

P21580

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 241-356 from the human protein was used as the immunogen for the A20 antibody.

Clonality

Monoclonal

Isotype

IgG2c κ

Clone

TNFAIP3/2813

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the A20 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This A20 antibody is available for research use only.

Storage Conditions

Store the A20 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the A20 antibody should be determined by the researcher.

Location

Cytoplasmic, Cell surface and Nuclear

Image Legend

IHC staining of FFPE human breast carcinoma with A20 antibody (clone TNFAIP3/2813) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.