Neurofilament Antibody Cocktail (Heavy + Light)
This MAb reacts with a 200kDa and 68kDa protein, identified as heavy and light sub-units of neurofilaments (NF-H & NF-L) . Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H) . Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/ million cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 minutes at RT
UniProt
P12036
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human neurofilament protein was used as the immunogen for the Neurofilament antibody cocktail.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
NF421 + NFL/736
Applications
FACS, IF, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the Neurofilament antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Neurofilament antibody cocktail is available for research use only.
Storage Conditions
Store the Neurofilament antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the Neurofilament antibody cocktail should be determined by the researcher.
Location
Cytoplasmic
Image Legend
Immunofluorescent staining of permeabilized human HEK293 cells with Neurofilament antibody cocktail (green) and Palloidin (red) .
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