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Recombinant Chromogranin A Antibody

Chromogranin A (CGA) is an acidic protein which is reported to be widely expressed in neural tissues and in secretory granules of human endocrine cells e.g. parathyroid gland, adrenal medulla, anterior pituitary gland, islet cells of the pancreas and C cells of the thyroid. GA is found in the large majority of epithelial NE tumors: pituitary adenomas, parathyroid and pancreatic islet cell tumors, medullary thyroid carcinoma, and carcinoid tumors. Together with synaptophysin, CGA is the most important marker for the identification of NE and neuronal tumors and NE/neuronal differentiation in other tumors.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

P10645

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 400-600 from the human protein was used as the immunogen for the recombinant Chromogranin A antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

CHGA/4544R

Type

Recombinant

Applications

IHC-P

Purity

Protein A affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the recombinant Chromogranin A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant Chromogranin A antibody is available for research use only.

Storage Conditions

Store the recombinant Chromogranin A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the recombinant Chromogranin A antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE human pancreas with recombinant Chromogranin A antibody (clone CHGA/4544R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

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