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Recombinant BrdU Antibody

It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

Not Applicable

Host

Mouse

Reactivity

All Species Expected

Immunogen

Bromodeoxyuridine conjugated to KLH was used as the immunogen for the recombinant BrdU antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG1 k

Clone

RBRD494

Type

Recombinant

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the recombinant BrdU antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant BrdU antibody is available for research use only.

Storage Conditions

Store the recombinant BrdU antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the recombinant BrdU antibody should be determined by the researcher.

Location

Nuclear

Image Legend

SDS-PAGE analysis of purified, BSA-free recombinant BrdU antibody (clone rBRD494) as confirmation of integrity and purity.

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