Recombinant BrdU Antibody
It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
Product Specifications
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT
UniProt
Not Applicable
Host
Mouse
Reactivity
All Species Expected
Immunogen
Bromodeoxyuridine conjugated to KLH was used as the immunogen for the recombinant BrdU antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG1 k
Clone
RBRD494
Type
Recombinant
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the recombinant BrdU antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This recombinant BrdU antibody is available for research use only.
Storage Conditions
Store the recombinant BrdU antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the recombinant BrdU antibody should be determined by the researcher.
Location
Nuclear
Image Legend
SDS-PAGE analysis of purified, BSA-free recombinant BrdU antibody (clone rBRD494) as confirmation of integrity and purity.
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