NSE Antibody / Neuron-specific enolase gamma
This monoclonal antibody recognizes a protein of about 50kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas.It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.
Product Specifications
CAS Number
9007-83-4
Specifications
ELISA: Use Ab at 2-4 µg/mL for coating; order Ab without BSA, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT
UniProt
P09104
Host
Mouse
Reactivity
Human
Immunogen
A portion of amino acids 416-433 from the human protein was used as the immunogen for the NSE antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
ENO2/2706
Applications
ELISA, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the NSE antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This NSE antibody is available for research use only.
Storage Conditions
Store the NSE antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the NSE antibody should be determined by the researcher.
Location
Cytoplasmic
Image Legend
Frequently Asked Questions
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