CD4 Antibody
CD4 is a member of the Ig superfamily, primarily expressed on most thymocytes, a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a coreceptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosine kinase, lck. CD4 is expressed by the majority of thymocytes, most helper T cells, a subset of NK-T cells and weakly by dendritic cells and macrophages. CD4 plays an important role in the development of T cells and is required for mature T cells to function optimally.This antibody blocks helper T cell responses to MHC class II antigens, including cytolysis, proliferation, allogeneic B cell help, and release of lymphokines.
Product Specifications
CAS Number
9007-83-4
Specifications
UniProt
P06332
Host
Rat
Reactivity
Mouse
Immunogen
Mouse CTL clone V4 was used as the immunogen for the CD4 antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
GK1.5
Applications
ELISA, Neutralization, FACS, IF
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the CD4 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This CD4 antibody is available for research use only.
Storage Conditions
Store the CD4 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the CD4 antibody should be determined by the researcher.
Location
Cell surface
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