IL-4 Antibody
IL-4 is a pleiotropic cytokine produced by activated T cells, mast cells, and basophils. IL-4 is a potent lymphoid cell growth factor, which stimulates the growth and activation of certain B cells and T cells. IL-4 is important for regulation of T helper subset development. IL-4 is expressed primarily by activated Th2 cells and NK cells, and at lower levels by mast cells, and basophils. IL-4 signals through the IL-4R, and dendritic cell IL-12.
Product Specifications
CAS Number
9007-83-4
Specifications
ELISA (order BSA/azide-free format), , Flow cytometry: 1-2ug/10^6 cells in 0.1ml, Immunofluorescence: 1-2 µg/mL
UniProt
P07750
Host
Rat
Reactivity
Mouse
Immunogen
Partially purified native mouse IL-4 protein was used as the immunogen for this IL4 antibody.
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
11B11
Applications
ELISA, FACS, IF
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the IL-4 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This IL-4 antibody is available for research use only.
Storage Conditions
Store the IL-4 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the IL-4 antibody should be determined by the researcher.
Image Legend
SDS-PAGE analysis of purified, BSA-free IL-4 antibody (clone 11B11) as confirmation of integrity and purity.
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