CD8b Antibody
This monoclonal antibody reacts with the mouse CD8 beta molecule. The CD8 beta chain associates with the CD8 alpha chain to form the CD8 alpha/beta heterodimer expressed on the surface of a majority of thymocytes, and on peripheral cytotoxic alpha beta TCR T cells. This monoclonal antibody identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing.
Product Specifications
CAS Number
9007-83-4
Specifications
Functional Studies (order BSA/azide-free format), Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 0.5-1 µg/mL
UniProt
P10300
Host
Rat
Reactivity
Mouse
Immunogen
Five day mixed leukocyte culture population, C57BL/6 anti-BALB/c
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
H35-17.2
Applications
Functional Studies, FACS, IF
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the CD8b antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This CD8b antibody is available for research use only.
Storage Conditions
Store the CD8b antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the CD8b antibody should be determined by the researcher.
Image Legend
SDS-PAGE analysis of purified, BSA-free CD8b antibody (clone H35-17.2) as confirmation of integrity and purity.
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