HPV16 E1/E4 Antibody

The human papilloma virus (HPV) family of DNA tumor viruses includes HPV16, a 'high-risk' sexually-transmitted HPV that can lead to cervical, anal, vulvar, head, neck, and penile cancer. The HPV16E1/E4 protein is expressed abundantly in cells supporting viral DNA amplification, but is lost during malignant progression. HPV16E1/E4 causes G2 cell cycle arrest by associating with and preventing the nuclear entry of Cdk1/cyclin B1 complexes. HPV16E1/E4 also interacts with cyclin A and Cdk2 during the G2 phase of the cell cycle, and this association may increase the efficiency with which HPV16E1/E4 is able to prevent mitotic entry. HPV16E1/E4 also associates with keratin intermediate filaments and causes the network to collapse.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

Host

Mouse

Reactivity

HPV-16

Immunogen

A recombinant human HPV16 E1/E4 fragment (amino acids 36-41) was used as the immunogen for this HPV16 E1/E4 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

HPV16 E1/E4

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the HPV16 E1/E4 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This HPV16 E1/E4 antibody is available for research use only.

Storage Conditions

Store the HPV16 E1/E4 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the HPV16 E1/E4 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC staining of FFPE human cervix with HPV16 E1/E4 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.