Melanoma Marker Antibody Cocktail (MART-1 & gp100)

This antibody cocktail recognizes two melanoma-specific proteins, which include MART-1 and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. A cocktail of these two markers is sensitive for labeling formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

Host

Mouse

Reactivity

Human

Immunogen

Recombinant hMART-1 protein (DT101 & BC199) and extract of pigmented melanoma metastases from lymph nodes (HMB45) were used as the immunogen for this Melanoma Marker antibody cocktail.

Clonality

Monoclonal

Isotype

IgG2b κ,IgG2a λ,IgG1 κ

Clone

DT101+ BC199 + HMB45

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the Melanoma Marker antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Melanoma Marker antibody cocktail is available for research use only.

Storage Conditions

Store the Melanoma Marker antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the Melanoma Marker antibody cocktail should be determined by the researcher.

Image Legend

IHC staining of FFPE human melanoma tissue with Melanoma Marker antibody cocktail (clones DT101+ BC199 + HMB45) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.