Cytokeratin 5/6/18 Antibody

It recognizes polypeptides of 58kDa, 56kDa, and 45kDa, identified as cytokeratin 5, 6, and 18 respectively. It shows no reaction with keratin 1, 8, or 19. This MAb shows a broad pattern of reactivity with epithelial tissues, from simple glandular epithelia to stratified squamous epithelia. Epithelial cells are labeled whether they are ectodermal, mesodermal, or endodermal in origin. This MAb is useful in detecting cells of epithelial origin in mixed tumors, in effusions, in bone marrow samples, or in tissue culture cells.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

Host

Mouse

Reactivity

Human

Immunogen

The detergent-insoluble fraction of psoriatic human epidermis was used as the immunogen for the Cytokeratin 5/6/18 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

SPM267

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the Cytokeratin 5/6/18 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Cytokeratin 5/6/18 antibody is available for research use only.

Storage Conditions

Store the Cytokeratin 5/6/18 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the Cytokeratin 5/6/18 antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human skin with Cytokeratin 5/6/18 antibody (clone SPM267) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.