Recombinant Pan-HLA Antibody (DP/DQ/DR)

Reacts with a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DP, -DQ and -DR. Human MHC class II antigens are transmembrane glycoproteins composed of an alpha chain (36kDa) and a beta chain (27kDa) . They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, and thymic epithelial cells and are also present on activated T lymphocytes. Human MHC class II genes are located in the HLA-D region that encodes at least six alpha and ten beta chain genes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They, therefore, have a critical role in the initiation of the immune response.

Product Specifications

CAS Number

9000-83-3

Specifications

Flow cytometry: 1-2ug/10^6 cells in 0.1ml, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 1-2 µg/mL

UniProt

P04440 (HLA-DP) ; P01908; P01909; P01920 (HLA-DQ) ; P01903 (HLA-DR)

Host

Mouse

Reactivity

Human

Immunogen

The non-T, non-B human acute lymphoblastic leukemia REH6 cell line was used as the immunogen for the Pan-HLA antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG1 k

Clone

RHLA-Pan/3475

Type

Recombinant

Applications

FACS, IF, IHC-P, WB

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the recombinant Pan-HLA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant Pan-HLA antibody is available for research use only.

Storage Conditions

Store the recombinant Pan-HLA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant Pan-HLA antibody should be determined by the researcher.

Location

Cell surface

Image Legend

Flow cytometry testing of human Raji cells with recombinant Pan-HLA antibody (clone rHLA-Pan/3475) ; Red=isotype control, Blue= recombinant Pan-HLA antibody.