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Bcl-X Antibody

Recognizes a protein of 27kDa, identified as the Bcl-X protein. This MAb shows no cross-reaction with Bcl-2 or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241 amino acid protein which suppresses cell death. And Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

Q07817

Host

Mouse

Reactivity

Human

Immunogen

A recombinant human partial protein (amino acids 1-135) was used as the immunogen for this Bcl-X antibody.

Clonality

Monoclonal

Isotype

IgG

Clone

SPM519

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the Bcl-X antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Bcl-X antibody is available for research use only.

Storage Conditions

Store the Bcl-X antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the Bcl-X antibody should be determined by the researcher.

Image Legend

IHC staining of FFPE human Hodgkin's lymphoma with Bcl-X antibody (clone SPM519) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

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