Cytokeratin 7/17 Antibody

It recognizes intermediate filament proteins (IFP's) of 55kDa and 46kDa, which are identified as Cytokeratin 7 and Cytokeratin 17 respectively. On formalin-fixed, paraffin embedded sections, C-46 reacts with Keratin 7 only. Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation, which is directly applicable to the characterization of malignant tumors. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Cytokeratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. Cytokeratin 17 is expressed in epithelial cells of various origins, such as bronchial epithelial cells and skin appendages. Cytokeratin 17 antibody marks basal cell differentiation.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL (CK7 + CK17), Immunohistochemistry (FFPE) : 1-2 µg/mL (CK7)

UniProt

P08729 , Q04695

Host

Mouse

Reactivity

Human

Immunogen

A cytoskeletal preparation from human HeLa cells was used as the immunogen for this Cytokeratin 7/17 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

C-46

Applications

IF, WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the Cytokeratin 7/17 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Cytokeratin 7/17 antibody is available for research use only.

Storage Conditions

Store the Cytokeratin 7/17 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the Cytokeratin 7/17 antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human lung small cell carcinoma with Cytokeratin 7/17 antibody (clone C-46) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.