Proliferation Marker Antibody

JC1 detects a nuclear antigen, distinct from Ki67, present in proliferating cells. It is useful as a marker of proliferation in cases such as squamous cell carcinoma where the Ki67 index cannot be determined.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

Host

Mouse

Reactivity

Human

Immunogen

Pokeweed-stimulated lymphocytes were used as the immunogen for this Proliferation Marker antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

JC1

Applications

WB, IF, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the Proliferation Marker antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Proliferation Marker antibody is available for research use only.

Storage Conditions

Store the Proliferation Marker antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the Proliferation Marker antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC staining of FFPE human lymph node with Proliferation Marker antibody (clone JC1) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.