Cig2 Antibody
Cig2 is a cyclin that functions as the catalytic subunit of cyclin-dependent kinases (Cdks) in the fission yeast Schizosaccharomyces pombe. It is a B-type, S phase cyclin that is required for both the G1/S and G2/M cell cycle transitions and is expressed in a sharp spike that peaks during the G1/S period. Cig2 binds to Cdc2 (Cdk), and the resulting Cdc2/Cig2 complex controls the G1/S transition of the cell cycle. Disruption of Cig2 delays the onset of mitosis. The expression of the Cig2 gene is dependent on Mlu1-binding factor (MBF), and the protein is destroyed during anaphase by the APC/cyclosome (APC/C) and Skp1/Cullin-1/F-box (SCF), thereby ensuring the spike expression pattern of Cig2. SCF regulates Cig2 levels in a dual manner, transcriptionally and post-translationally, while APC/C only destroys the protein.
Product Specifications
CAS Number
9007-83-4
Specifications
ELISA (order BSA-free format for coating), Western blot: 1-2 µg/mL
UniProt
P36630
Host
Mouse
Immunogen
Bacterial S. pombe Cig2 purified protein was used as the immunogen for the Cig2 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
3A11/5
Applications
ELISA, WB
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the Cig2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Cig2 antibody is available for research use only.
Storage Conditions
Store the Cig2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the Cig2 antibody should be determined by the researcher.
Frequently Asked Questions
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