HA Tag Antibody

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA (order BSA-free format for coating), Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL, Flow cytometry: 1-2ug/million cells, Immunohistochemistry (FFPE) : 1-2 µg/mL

Host

Mouse

Immunogen

Amino acids YPYDVPDYA were used as the immunogen for this HA Tag antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

16.43

Applications

WB, IF, IHC-P, FACS, ELISA

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the HA Tag antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This HA Tag antibody is available for research use only.

Storage Conditions

Store the HA Tag antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the HA Tag antibody should be determined by the researcher.

Image Legend

Immunofluorescent staining of PFA-fixed human MCF7 cells that have been transfected with an HA-tagged protein, with HA Tag antibody (clone 16.43, green) and Reddot nuclear stain (red) .