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Recombinant Bromodeoxyuridine Antibody / BrdU

It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

Host

Mouse

Reactivity

All Species Expected

Immunogen

Bromodeoxyuridine conjugated to BSA was used as the immunogen for the BrdU antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG1 k

Clone

RBRD.3

Type

Recombinant

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the recombinant Bromodeoxyuridine antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant Bromodeoxyuridine antibody is available for research use only.

Storage Conditions

Store the recombinant Bromodeoxyuridine antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant Bromodeoxyuridine antibody should be determined by the researcher.

Location

Nuclear

Image Legend

SDS-PAGE analysis of purified, BSA-free recombinant Bromodeoxyuridine antibody (clone rBRD.3) as confirmation of integrity and purity.

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