Recombinant Bromodeoxyuridine Antibody / BrdU
It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
Product Specifications
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL
Host
Mouse
Reactivity
All Species Expected
Immunogen
Bromodeoxyuridine conjugated to BSA was used as the immunogen for the BrdU antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG1 k
Clone
RBRD.3
Type
Recombinant
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the recombinant Bromodeoxyuridine antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This recombinant Bromodeoxyuridine antibody is available for research use only.
Storage Conditions
Store the recombinant Bromodeoxyuridine antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the recombinant Bromodeoxyuridine antibody should be determined by the researcher.
Location
Nuclear
Image Legend
SDS-PAGE analysis of purified, BSA-free recombinant Bromodeoxyuridine antibody (clone rBRD.3) as confirmation of integrity and purity.
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