RET Antibody / c-RET

The Ret proto-oncogene is structurally related to the growing family of tyrosine kinase transmembrane receptors and is involved in GDNF signaling. RET expression is reported in several regions of the central nervous system; in the developing cranial nerve ganglia and a subset of cells within dorsal root ganglia, in motor neurons in the spinal cord and hindbrain, in neuro-retina and the growing tips of the renal collecting ducts in developing kidney.Alterations in RETgene are associated with diseases including papillary thyroid carcinoma, multiple endocrine neoplasia (type 2A and 2B), familial medullary thyroid carcinoma, and a congenital developmental disorder known as Hirschsprung's disease.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA (order BSA-free format for coating), Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P07949

Host

Mouse

Reactivity

Human

Immunogen

A recombinant human partial protein (amino acids 702-848) was used as the immunogen for this RET antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

RET/2663

Applications

ELISA, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the RET antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This RET antibody is available for research use only.

Storage Conditions

Store the RET antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the RET antibody should be determined by the researcher.

Image Legend

IHC staining of FFPE human prostate carcinoma with RET antibody (clone RET/2663) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.