Cytokeratin 20 Antibody / CK20

This MAb recognizes an intermediate filament protein of 46kDa, identified as cytokeratin 20 (KRT20) . KRT20 is abundantly expressed in goblet cells and enterocytes of the gastrointestinal tract. It is a useful marker of pancreatic and colorectal cancer. KRT20 is expressed under normal, hyperplastic and neoplastic conditions.It has been detected in adenocarcinomas of the colon, stomachand biliary tract. Breast carcinomas are generally non-reactive.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 0.1-0.2 µg/mL, Western blot: 1-2 µg/mL

UniProt

P35900

Host

Mouse

Reactivity

Human

Immunogen

A recombinant human partial protein (amino acids 196-323) was used as the immunogen for this Cytokeratin 20 antibody.

Clonality

Monoclonal

Isotype

IgG κ

Clone

KRT20/3145

Applications

IHC-P, WB

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the Cytokeratin 20 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Cytokeratin 20 antibody is available for research use only.

Storage Conditions

Store the Cytokeratin 20 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the Cytokeratin 20 antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human colon carcinoma with Cytokeratin 20 antibody (clone KRT20/3145) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.