CLEC9A Antibody / DNGR-1
This mAb recognizes mouse DNGR-1, which is also known as CLEC9A, which is a highly specific marker of the CD8a+ and the CD103+ DC subsets, as well as a receptor able to recognize a preformed signal exposed on necrotic cells. In peripheral blood, it is highly restricted on the surface of BDCA31 (+) dendritic cells and on a small subset of CD14 (+) and CD16 (-) monocytes.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/10^6 cells in 0.1ml, Western blot: 1-2 µg/mL, Immunofluorescence: 1-2 µg/mL
UniProt
Q8RU4
Host
Rat
Reactivity
Mouse
Immunogen
RBL-2H3 cells expressing mouse CLEC9A fused to an HA epitope.
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
1F6
Applications
FACS, WB, IF
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the CLEC9A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This CLEC9A antibody is available for research use only.
Storage Conditions
Store the CLEC9A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the CLEC9A antibody should be determined by the researcher.
Image Legend
SDS-PAGE analysis of purified, BSA-free CLEC9A antibody (clone 1F6) as confirmation of integrity and purity.
Frequently Asked Questions
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