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VEGFR2 Antibody / VEGF Receptor 2 / KDR

This MAb is specific to Mouse VEGFR2/FLK-1/CD309 and does not cross-react with FLK-2. VEGFR2 is a type I transmembrane glycoprotein. It is a member of the CSF-1/PDGF receptor family of type III tyrosine kinase receptors. Endothelial cells, embryonic tissues, and megakaryocytes mainly express VEGFR2. It plays an important role in the regulation of angiogenesis, vasculogenesis, and vascular permeability. The ligands of VEGFR2 include VEGF-A, VEGF-C, VEGF-D, and VEGF splice isoforms. Ligation of VEGFR2 with its ligands results in the receptor dimerization and auto-phosphorylation, stimulating endothelial cell proliferation and migration.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot (Non-reducing) : 2-5 µg/mL, Blocks binding of VEGF to VEGF-R2, Inhibits VEGF-induced signalingformat), Blocks tumor growth in mice

UniProt

P35918

Host

Rat

Reactivity

Mouse

Immunogen

Recombinant full-length Mouse VEGFR2 protein was used as the immunogen for this VEGFR2 antibody.

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

DC101

Applications

WB, Neutralizing, Blocking, Inhibition

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the VEGFR2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This VEGFR2 antibody is available for research use only.

Storage Conditions

Store the VEGFR2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the VEGFR2 antibody should be determined by the researcher.

Image Legend

SDS-PAGE analysis of purified, BSA-free VEGFR2 antibody (clone DC101) as confirmation of integrity and purity.

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