CD3e Antibody
CD3 chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex. The 145-2C11 antibody is useful for in vitro blocking and activation assays, as well as apoptosis induction and in vivo T cell depletion.
Product Specifications
CAS Number
9007-83-4
Specifications
Functional Studies (order BSA/azide-free format), Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL
UniProt
P07766
Reactivity
Mouse
Immunogen
The H2Kb-specific mouse cytotoxic T lymphocyte clone BM10 37 was used as the immunogen for the CD3e antibody.
Clonality
Monoclonal
Isotype
IgG κ
Clone
145-2C11
Applications
Functional Studies, FACS, IF
Purity
Protein A affinity chromatography
Format
Purified
Buffer
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the CD3e antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This CD3e antibody is available for research use only.
Storage Conditions
Store the CD3e antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the CD3e antibody should be determined by the researcher.
Location
Cell surface, cytoplasmic
Image Legend
SDS-PAGE analysis of purified, BSA-free CD3e antibody (clone 145-2C11) as confirmation of integrity and purity.
Documents
Frequently Asked Questions
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