Recombinant CD74 / CL Antibody
It recognizes a protein of ~35kDa, identified as CD74 (Workshop IV) . CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+) .
Product Specifications
CAS Number
9000-83-3
Specifications
Flow cytometry: 1-2ug/10^6 cells in 0.1ml, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P04233
Host
Rabbit
Reactivity
Human
Immunogen
SU-DHL-4 lymphoma cells were used as the immunogen for this recombinant CD74 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG κ
Clone
CLIP/3127R
Type
Recombinant
Applications
FACS, WB, IHC-P
Purity
Protein A affinity chromatography
Format
Purified
Buffer
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the recombinant CD74 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This recombinant CD74 antibody is available for research use only.
Storage Conditions
Store the recombinant CD74 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the recombinant CD74 antibody should be determined by the researcher.
Location
Cell surface and paranuclear globular
Image Legend
Western blot testing of human Raji cell lysate with recombinant CD74 antibody (clone CLIP/3127R) . Expected molecular weight: 33-43 kDa.
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