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Recombinant CD68 Antibody

This antibody recognizes a glycoprotein of 110kDa, which is identified as CD68. It is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. It reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Lymphomas are negative or show few granules.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P34810

Host

Mouse

Reactivity

Human

Immunogen

A recombinant full-length human protein was used as the immunogen for this recombinant CD68 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG1 k

Clone

RLAMP4/824

Type

Recombinant

Applications

IF, WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the recombinant CD68 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant CD68 antibody is available for research use only.

Storage Conditions

Store the recombinant CD68 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the recombinant CD68 antibody should be determined by the researcher.

Location

Cell surface, cytoplasmic

Image Legend

Immunofluorescent staining of permeabilzed human U-87 MG cells with recombinant CD68 antibody (clone rLAMP4/824, green) and Reddot nuclear stain (red) .

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