Recombinant CD44 Antibody / HCAM
Recognizes a cell surface glycoprotein of 80-95kDa (CD44) on lymphocytes, monocytes, and granulocytes (Leucocyte Typing Workshop V) . Its epitope is resistant to digestion by trypsin and chymotrypsin. This MAb selectively interferes with lymphocyte binding to lymph node, mucosal and synovial endothelium. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95kDa or hematopoietic variant (CD44s) . Higher molecular weight isoforms are described in epithelial cells (CD44v), which are believed to function in intercellular adhesion and stromal binding. CD44 immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium.
Product Specifications
CAS Number
9000-83-3
Specifications
Western blot: 1-2 µg/mL, Flow cytometry: 1-2ug/10^6 cells in 0.1ml, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P16070
Host
Mouse
Reactivity
Human
Immunogen
A recombinant full-length human CD44 protein was used as the immunogen for this recombinant CD44 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG1 k
Clone
RHCAM/918
Type
Recombinant
Applications
WB, FACS, IF, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the recombinant CD44 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This recombinant CD44 antibody is available for research use only.
Storage Conditions
Store the recombinant CD44 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the recombinant CD44 antibody should be determined by the researcher.
Image Legend
Western blot testing of human HeLa cell lysate with recombinant CD44 antibody (clone rHCAM/918) . Predicted molecular weight ~81 kDa.
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