CD22 Antibody
Recognizes a protein of 130-140kDa, identified as CD22 (also known as BL-CAM) . CD22 expression is restricted to normal and neoplastic B cells and is absent from other haemopoietic cell types. In B-cell ontogeny, CD22 is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD+. It is not expressed by plasma cells, CD22 is found highly expressed in follicular mantle and marginal zone B-cells, and while germinal center B-cells are relatively weak. CD22 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/10^6 cells in 0.1ml
UniProt
P20273
Host
Mouse
Reactivity
Human
Immunogen
Human tonsil lymphocytes were used as the immunogen for the CD22 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
RFB4
Applications
FACS
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Store the CD22 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This CD22 antibody is available for research use only.
Storage Conditions
Store the CD22 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
Optimal dilution of the CD22 antibody should be determined by the researcher.
Image Legend
Flow cytometry testing of human Ramos cells with CD22 antibody (clone RFB4) ; Red=isotype control, Blue= CD22 antibody.
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