Recombinant CD14 Antibody

Recognizes a protein of 55kDa, identified as CD14 (also known lipopolysaccharide receptor) . CD14 is expressed strongly on monocytes and macrophage and weakly on the surface of neutrophils. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP) . Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P08571

Host

Mouse

Reactivity

Human

Immunogen

A recombinant human partial protein (amino acids 25-148) was used as the immunogen for this recombinant CD14 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG1 k

Clone

RLPSR/2408

Type

Recombinant

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the recombinant CD14 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant CD14 antibody is available for research use only.

Storage Conditions

Store the recombinant CD14 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant CD14 antibody should be determined by the researcher.

Location

Cell surface, Secreted, Cytoplasmic (Golgi)

Image Legend

IHC staining of FFPE human spleen with recombinant CD14 antibody (clone rLPSR/2408) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.