GCLM Antibody

GCLM, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

P48507

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human full-length GCLM protein was used as the immunogen for the GCLM antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

CPTC-GCLM-1

Applications

WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the GCLM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This GCLM antibody is available for research use only.

Storage Conditions

Store the GCLM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the GCLM antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE human tonsil with GCLM antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.