GCLM Antibody

GCLM, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

P48507

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human full-length GCLM protein was used as the immunogen for the GCLM antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

CPTC-GCLM-1

Applications

WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the GCLM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This GCLM antibody is available for research use only.

Storage Conditions

Store the GCLM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the GCLM antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE human tonsil with GCLM antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.