Recombinant EpCAM Antibody

EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM) . It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. Antibody to Ep-CAM has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Product Specifications

CAS Number

9000-83-3

Specifications

Flow cytometry: 1-2ug/10^6 cells in 0.1ml, Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P16422

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant human partial protein (amino acids 100-224) was used as the immunogen for the EpCAM antibody. The epitope of this mAb has been mapped to between amino acids 202-212.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

EGP40/2571R

Type

Recombinant

Applications

FACS, IHC-P

Purity

Protein A affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the recombinant EpCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant EpCAM antibody is available for research use only.

Storage Conditions

Store the recombinant EpCAM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant EpCAM antibody should be determined by the researcher.

Location

Cell surface, cytoplasmic

Image Legend

Flow cytometry testing of human MCF7 cells with recombinant EpCAM antibody (clone EGP40/2571R) ; Red=isotype control, Blue= recombinant EpCAM antibody.