CD8a Antibody
CD8 is a cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha) . A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class 1 and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells. For mature T-cells, CD4 and CD8 are mutually exclusive, so anti-CD8, generally used in conjunction with anti-CD4. It is a useful marker for distinguishing helper/inducer T-lymphocytes, and most peripheral T-cell lymphomas are CD4+/CD8-. Anaplastic large cell lymphoma is usually CD4+ and CD8-, and in T-lymphoblastic lymphoma/leukemia, CD4 and CD8 are often co-expressed. CD8 is also found in littoral cell angioma of the spleen.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/10^6 cells in 0.1ml
UniProt
P01732
Host
Mouse
Reactivity
Human
Immunogen
Thymocytes followed by Sezary T cells were used as the immunogen for the CD8a antibody.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
UCHT4
Applications
FACS
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Store the CD8a antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This CD8a antibody is available for research use only.
Storage Conditions
Store the CD8a antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Product Datasheet
https://www.nsjbio.com/tds-pdf/cd8a-antibody-ucht4-v8216
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
Optimal dilution of the CD8a antibody should be determined by the researcher.
Location
Cell surface
Image Legend
Flow cytometry testing of human Jurkat cells with CD8a antibody (clone UCHT4) ; Red=isotype control, Blue= CD8a antibody.
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