Alpha Actinin 4 Antibody / ACTN4
The spectrin gene family encodes a diverse group of cytoskeletal proteins that include spectrins, dystrophins and -actinins. There are four tissue-specific -actinins, namely -actinin-1, -actinin-2, -actinin-3 and -actinin-4, which are localized to muscle and non-muscle cells, including skeletal, cardiac and smooth muscle cells, as well as within the cytoskeleton. Each -actinin protein contains one Actin-binding domain, two calponin-homology domains, two EF-hand domains and four spectrin repeats, through which they function as bundling proteins that can cross-link F-Actin, thus anchoring Actin to a variety of intracellular structures. Defects in the gene encoding -actinin-4 are the cause of focal segmental glomerulosclerosis 1 (FSGS1), a common renal lesion characterized by decreasing kidney function and, ultimately, renal failure.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
O43707
Host
Mouse
Reactivity
Human
Immunogen
A recombinant full-length human ACTN4 protein was used as the immunogen for this Alpha Actinin 4 antibody.
Clonality
Monoclonal
Isotype
IgG
Clone
93
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the Alpha Actinin 4 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Alpha Actinin 4 antibody is available for research use only.
Storage Conditions
Store the Alpha Actinin 4 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the Alpha Actinin 4 antibody should be determined by the researcher.
Location
Nuclear, cytoplasmic
Image Legend
SDS-PAGE analysis of purified, BSA-free Alpha Actinin 4 antibody (clone 93) as confirmation of integrity and purity.
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