TOP1MT Antibody

DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

Q969P6

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human TOP1MT protein was used as the immunogen for the TOP1MT antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

TOP1MT/613

Applications

WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the TOP1MT antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This TOP1MT antibody is available for research use only.

Storage Conditions

Store the TOP1MT antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the TOP1MT antibody should be determined by the researcher.

Location

Cytoplasmic (Mitochondria)

Image Legend

IHC staining of FFPE human melanoma with TOP1MT antibody (clone TOP1MT/613) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.