Recombinant Thymidylate Synthase Antibody
It recognizes a protein of 36kDa, identified as Thymidylate Synthase (TS) (EC 2.1.1.45) . It converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein has been reported to associate with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.
Product Specifications
CAS Number
9000-83-3
Specifications
Flow cytometry: 1-2ug/million cells in 0.1ml, Immunofluorescence: 1-2 µg/mL
UniProt
P04818
Host
Mouse
Reactivity
Human
Immunogen
A recombinant human partial protein (amino acids 60-174) was used as the immunogen for the recombinant Thymidylate Synthase antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG1 k
Clone
RTYMS/1884
Type
Recombinant
Applications
FACS, IF
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Store the recombinant Thymidylate Synthase antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This recombinant Thymidylate Synthase antibody is available for research use only.
Storage Conditions
Store the recombinant Thymidylate Synthase antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
Optimal dilution of the recombinant Thymidylate Synthase antibody should be determined by the researcher.
Location
Nuclear, cytoplasmic
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