Recombinant Thymidylate Synthase Antibody

It recognizes a protein of 36kDa, identified as Thymidylate Synthase (TS) (EC 2.1.1.45) . It converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein has been reported to associate with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.

Product Specifications

CAS Number

9000-83-3

Specifications

Flow cytometry: 1-2ug/million cells in 0.1ml, Immunofluorescence: 1-2 µg/mL

UniProt

P04818

Host

Mouse

Reactivity

Human

Immunogen

A recombinant human partial protein (amino acids 60-174) was used as the immunogen for the recombinant Thymidylate Synthase antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG1 k

Clone

RTYMS/1884

Type

Recombinant

Applications

FACS, IF

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the recombinant Thymidylate Synthase antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant Thymidylate Synthase antibody is available for research use only.

Storage Conditions

Store the recombinant Thymidylate Synthase antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant Thymidylate Synthase antibody should be determined by the researcher.

Location

Nuclear, cytoplasmic

Image Legend

Flow cytometry testing of PFA-fixed human MOLT4 cells with recombinant Thymidylate Synthase antibody (clone rTYMS/1884) ; Red=isotype control, Blue= recombinant Thymidylate Synthase antibody.