Recombinant CD61 Antibody / Integrin beta 3 / ITGB3

Reacts with human integrin beta3 (GPIIIa, vitronectin receptor beta chain) . It associates with the IIb-chain (CD41) to form the GpIIb/GpIIIa complex (CD41/CD61) .The CD41/CD61 complex appears early in megakaryocyte maturation. The activated CD41/CD61 complex is a receptor for von Willebrand factor, soluble fibrinogen, fibronectin, vitronectin and thrombospondin. It plays a central role in platelet activation and aggregation. The CD51/CD61 is implicated in tumor metastasis and adenoviral infection. The antibody detects platelets in smears of blood and bone marrow, as well as megakaryocytes in frozen sections and cell smears. The antibody is useful for classification of megakaryoblastic leukemia.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P05106

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant human partial protein (amino acids 385-490) was used as the immunogen for this recombinant CD61 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

ITGB3/3126R

Type

Recombinant

Applications

IHC-P

Purity

Protein A affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the recombinant CD61 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant CD61 antibody is available for research use only.

Storage Conditions

Store the recombinant CD61 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the recombinant CD61 antibody should be determined by the researcher.

Location

Cell surface, cytoplasmic

Image Legend

IHC testing of FFPE human spleen with recombinant CD61 antibody (ITGB3/3126R) . Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.