Recombinant GRP94 Antibody / HSP90B1

Recognizes a protein of 94kDa, which is identified as the glucose-regulated protein 94 (grp94) and also tumor rejection antigen (gp96) . Grp94 shows a high degree of sequence homology with the heat shock protein 90 (hsp90) . This MAb is highly specific to grp94 and shows minimal cross-reaction with other members of the HSP90 family. Grp's are a class of proteins unresponsive to heat shock and are induced by glucose deprivation. Grp94 has been briefly studied as a prognostic factor in breast cancer.

Product Specifications

CAS Number

9000-83-3

Specifications

Flow cytometry: 1-2ug/10^6 cells, Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P14625

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant full-length human protein was used as the immunogen for this recombinant GRP94 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

HSP90B1/3168R

Type

Recombinant

Applications

FACS, IHC-P

Purity

Protein A affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the recombinant GRP94 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant GRP94 antibody is available for research use only.

Storage Conditions

Store the recombinant GRP94 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant GRP94 antibody should be determined by the researcher.

Location

Nuclear, cytoplasmic

Image Legend

Flow cytometry testing of PFA-fixed human HepG2 cells with recombinant GRP94 antibody (clone HSP90B1/3168R) ; Red=isotype control, Blue= recombinant GRP94 antibody.