CD11b Antibody / MAC-1

Integrin ITGAM/ITGB2/CD11b/MAC-1 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P11215

Host

Mouse

Reactivity

Human

Immunogen

Amino acids 941-1074 were used as the immunogen for this CD11b antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

CDLA11b-1

Applications

WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the CD11b antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This CD11b antibody is available for research use only.

Storage Conditions

Store the CD11b antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the CD11b antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cell membrane, cytoplasm

Image Legend

IHC staining of FFPE human spleen with CD11b antibody (clone CDLA11b-1) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.