P53 Antibody / TP53
Recognizes a 53kDa protein, which is identified as p53 suppressor gene product. It reacts with the mutant as well as the wild form of p53. Its epitope maps within the N-terminus (aa 20-25) of p53. Monoclonal antibody PAb1801 does not block the binding of DO-7 MAb to p53 in an ELISA test. p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia. Mutations involving p53 are found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/10^6 cells in 0.1ml, Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 0.25-0.5 µg/mL
UniProt
P04637
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human wild type p53 protein expressed in E. coli was used as the immunogen for the p53 antibody.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
DO-1
Applications
FACS, IF, WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the p53 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This p53 antibody is available for research use only.
Storage Conditions
Store the p53 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the p53 antibody should be determined by the researcher.
Location
Nuclear
Image Legend
Western blot testing of human HeLa cell lysate with p53 antibody (DO-1) . Expected molecular weight ~53 kDa.
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