Recombinant dsDNA Antibody

Double Stranded deoxyribonucleic acid (ds DNA) is the genetic material of all cells and many viruses and is a polymer of nucleotides. The monomer consists of phosphorylated 2-deoxyribose N-glycosidically linked to one of four bases, adenine, cytosine, guanine or thymine. These are linked together by 3',5'-phosphodiester bridges. In the Watson-Crick double-helix model, two complementary strands are wound in a right-handed helix and held together by hydrogen bonds between complementary base pairs.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

Not Known

Host

Mouse

Reactivity

Human

Immunogen

Nuclei of Burkitt's cells were used as the immunogen for the recombinant dsDNA antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

DSD/4054R

Type

Recombinant

Applications

IHC-P

Purity

Protein A affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the recombinant dsDNA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant dsDNA antibody is available for research use only.

Storage Conditions

Store the recombinant dsDNA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant dsDNA antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC: Formalin-fixed, paraffin-embedded human colon carcinoma stained with recombinant dsDNA antibody (clone DSD/4054R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.