GMCSF Antibody

Granulocyte/macrophage - Colony-stimulating factor (GM-CSF) is a hematopoietic factor that is produced by activated T-cells, B-cells, mast cells, macrophages, fibroblasts, and endothelial cells. In addition to supporting colony formation of granulocyte/macrophage progenitors, GM-CSF is a growth factor for erythroid, megakaryocyte, and eosinophil progenitors.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P04141

Host

Rat

Reactivity

Human

Immunogen

Recombinant human protein was used as the immunogen for this GMCSF antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

CSF2/3403

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the GMCSF antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This GMCSF antibody is available for research use only.

Storage Conditions

Store the GMCSF antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the GM-CSF antibody to be titered up or down for optimal performance.

Location

Secreted (extracellular)

Image Legend

IHC staining of FFPE human spleen with GMCSF antibody (clone CSF2/3403) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.