SPTB Antibody / Spectrin beta
Spectrin is a cytoskeletal protein that lines the intracellular side of the plasma membrane of many cell types in pentagonal or hexagonal arrangements, forming a scaffolding and playing an important role in maintenance of plasma membrane integrity and cytoskeletal structure. In certain types of brain injury such as diffuse axonal injury, spectrin is irreversibly cleaved by the proteolytic enzyme calpain, destroying the cytosketelon. Spectrin cleavage causes the membrane to form blebs and ultimately to be degraded, usually leading to cell death.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P11277
Host
Mouse
Reactivity
Human
Immunogen
Red cell membranes were used as the immunogen for this Spectrin beta antibody.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
RG/26
Applications
WB, IF, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the SPTB antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This SPTB antibody is available for research use only.
Storage Conditions
Store the SPTB antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the SPTB antibody should be determined by the researcher.
Image Legend
SDS-PAGE analysis of purified, BSA-free SPTB antibody (clone RG/26) as confirmation of integrity and purity.
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