BMI1 Antibody
The B cell-specific moloney murine leukemia virus integration site 1 (Bmi-1) is a transcriptional receptor of the polycomb gene family involved in several cellular processes including cell-cycle regulation, apoptosis, and maintenance of adult and neoplastic stem cells by providing self-renewal capacity. Further, Bmi-1 expression has been associated with malignant transformation, tumor progression, metastatic disease, and poor prognosis in human malignancies.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P35226
Host
Mouse
Reactivity
Human, Mouse
Immunogen
A recombinant human partial protein (amino acids 142-326) was used as the immunogen for this BMI1 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
BMI1/2823
Applications
WB, IF, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the BMI1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This BMI1 antibody is available for research use only.
Storage Conditions
Store the BMI1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the BMI1 antibody should be determined by the researcher.
Location
Nuclear
Image Legend
Western blot testing of mouse NIH3T3 cell lysate with BMI1 antibody. Predicted molecular weight: 37-43 kDa.
Frequently Asked Questions
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