L-Selectin Antibody / CD62L
Selectins, also designated CD62 antigens, comprise a family of carbohydrate-binding proteins involved in mediating cellular interactions with leukocytes. L-Selectin (also designated LECAM-1 or CD62L) is expressed on the majority of B and naive T cells and on most monocytes, neutrophils and eosinophils. L-Selectin interacts with specific carbohydrates expressed by activated endothelial cells. P-Selectin (also designated GMP-140 or CD62P), expressed on activated platelets and endothelial cells, and E-Selectin (also designated ELMA-1 or CD62E), expressed on endothelial cells, exhibit overlapping ligand specificities. Both recognize sialyl-Le (x) as a ligand and bind to specific carbohydrates on neutrophils and monocytes.
Product Specifications
CAS Number
9007-83-4
Specifications
ELISA (order BSA-free format for coating), Flow cytometry: 1-2ug/10^6 cells in 0.1ml, Western blot: 1-2 µg/mL
UniProt
P14151
Host
Mouse
Reactivity
Human
Immunogen
Supernatant from phorbol myristic acid activated human peripheral blood leukocytes was used as the immunogen for the L-Selectin antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
LAM1-116
Applications
ELISA, FACS, WB
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the L-Selectin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This L-Selectin antibody is available for research use only.
Storage Conditions
Store the L-Selectin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the L-Selectin antibody should be determined by the researcher.
Image Legend
SDS-PAGE analysis of purified, BSA-free L-Selectin antibody (clone LAM1-116) as confirmation of integrity and purity.
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