CHP2 Antibody / Calcineurin B homologous protein 2
Selected Variant
V8074-100UG
Size: 100 µg
CHP2 (calcineurin B homologous protein 2), also known as hepatocellular carcinoma-associated antigen 520, is a 196 amino acid protein that contains 4 EF-hand domains and plays a potential role in transmembrane Na+/H+ exchange. By binding to and activating NHE-1, CHP2 increases the pH and works to protect cells from serum deprivation-induced death. Though not typically detected in normal tissues, CHP2 is highly expressed in malignantly transformed cells and is therefore considered to be a tumor-associated antigen. Ectopic expression of CHP2 promotes proliferation of HEK293 cells and knockdown of CHP2 mRNA in HepG2 cells inhibits cell proliferation. Like calcineurin B, CHP2 can bind to and stimulate phosphatase activity of calcineurin A and activate the calcineurin/NFAT signaling pathway.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunofluorescence: 1-2 µg/mL
UniProt
O43745
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human full-length protein was used as the immunogen for the CHP2 antibody.
Clonality
Monoclonal
Isotype
IgG2c κ
Clone
CPTC-CHP2-1
Applications
WB, IF
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the CHP2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This CHP2 antibody is available for research use only.
Storage Conditions
Store the CHP2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the CHP2 antibody should be determined by the researcher.
Location
Nuclear, cytoplasmic
Image Legend
Immunofluorescent staining of permeabilized human MCF7 cells with CHP2 antibody (green) and Reddot nuclear stain (red) .
Price
Documents
Frequently Asked Questions
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