Replication Protein A2 Antibody / RPA32
Recognizes a protein of 34kDa, identified as a subunit of Replication Protein A (RPA) (also known as human single-stranded DNA binding protein, or HSSB) . RPA from human cells is a stable heterotrimer of 70kDa, 32-34kDa, and 11-14kDa subunits (RPA70, RPA32, and RPA14 respectively) . It is involved in DNA replication, repair, and recombination. RPA is required for the SV40 large tumor antigen-catalyzed unwinding of SV40 DNA and stimulates DNA polymerase (pol) alpha and delta. RPA34 is phosphorylated at the G1/S boundary of the cell cycle or upon exposure of cells to DNA damage-inducing agents including ionizing and UV radiation.
Product Specifications
Specifications
Western blot: 1-2 µg/mL
UniProt
P15927
Host
Mouse
Reactivity
Human
Immunogen
A recombinant human full-length protein was used as the immunogen for this Replication Protein A2 antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
SPM316
Applications
WB
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the Replication Protein A2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Replication Protein A2 antibody is available for research use only.
Storage Conditions
Store the Replication Protein A2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Product Datasheet
https://www.nsjbio.com/tds-pdf/replication-protein-a2-antibody-rpa32-spm316-v8066
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the Replication Protein A2 antibody should be determined by the researcher.
CAS Number
9007-83-4
Image Legend
Western blot testing of human samples with Replication Protein A2 antibody (clone SPM316) . Expected molecular weight ~32 kDa.
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