Replication Protein A2 Antibody / RPA32
Recognizes a protein of 34kDa, identified as a subunit of Replication Protein A (RPA) (also known as human single-stranded DNA binding protein, or HSSB) . RPA from human cells is a stable heterotrimer of 70kDa, 32-34kDa, and 11-14kDa subunits (RPA70, RPA32, and RPA14 respectively) . It is involved in DNA replication, repair, and recombination. RPA is required for the SV40 large tumor antigen-catalyzed unwinding of SV40 DNA and stimulates DNA polymerase (pol) alpha and delta. RPA34 is phosphorylated at the G1/S boundary of the cell cycle or upon exposure of cells to DNA damage-inducing agents including ionizing and UV radiation.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL
UniProt
P15927
Host
Mouse
Reactivity
Human
Immunogen
A recombinant human full-length protein was used as the immunogen for this Replication Protein A2 antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
SPM316
Applications
WB
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the Replication Protein A2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Replication Protein A2 antibody is available for research use only.
Storage Conditions
Store the Replication Protein A2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Product Datasheet
https://www.nsjbio.com/tds-pdf/replication-protein-a2-antibody-rpa32-spm316-v8066
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the Replication Protein A2 antibody should be determined by the researcher.
Image Legend
Western blot testing of human samples with Replication Protein A2 antibody (clone SPM316) . Expected molecular weight ~32 kDa.
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