RORC Antibody

This MAb recognizes a protein of 63kDa, identified as ROR-C. Its epitope maps in between aa1-50. The nuclear orphan receptors ROR and ROR are members of the nuclear hormone receptor superfamily. Members of this family act by directly associating with DNA sequences known as hormone response elements (HREs) and typically bind DNA as either homo- or heterodimers. RORalpha and RORgamma are unique in that they bind DNA as monomers. RORalpha has multiple isoforms that share common DNA and putative ligand-binding domains, but differ in their amino terminal domains, which are generated by alternative RNA processing. RORgamma comprises a 560 amino acid protein that shares 50% amino acid identity with RORalpha and is most highly expressed in skeletal muscle. Although these proteins are considered orphan receptors, due to a lack of defined ligands, experimental evidence has shown that melatonin may be the natural ligand for these nuclear receptors.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA (order BSA-free format for coating)

UniProt

P51449

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human RORC protein was used as the immunogen for the RORC antibody. The epitope has been found to be with amino acids 1-50.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

RORC/2942

Applications

ELISA

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the RORC antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This RORC antibody is available for research use only.

Storage Conditions

Store the RORC antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the RORC antibody should be determined by the researcher.

Image Legend

Analysis of HuProt (TM) microarray containing more than 19,000 full-length human proteins using RORC antibody (clone RORC/2942) . These results demonstrate the foremost specificity of the RORC/2942 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.